Gene sets which were significantly different between WT and Rag1−/− mice after stroke revealed that the absence of lymphocytes significantly reduces microglial genes associated with macrophage activation state (G6: Foxp1, SykShi et al., 2008; Tabata et al., 2020), whereas genes associated with cytokine/chemokine stimulus were enriched (G3: Il1b, Tnf, Csf1, and Ccl2) in Rag1−/− in comparison to WT microglia (Figure 2F). Here, TNF is linked to stroke disorder.