When we compared the stroke-associated microglial genes between WT and Rag1−/− mice, we found that 67 genes – including the majority of the DAM signature (Apoe, B2m, Cstb, Lgals3, Lyz2, and Spp1) – were not specific to the lymphocyte deficiency (Figure 2E), whereas 120 stroke-associated microglial genes were only present in mice lacking lymphocytes, such as genes involved in cytokine signaling and chemotaxis (i.e. Cd74, Ccl2, Ccl7, H2-Ab1, Infgr1, Mif, Pf4, and Tnf). This evidence concerns the gene SPP1 and stroke disorder.