As shown in Fig. 4A–E, TLR4, phospho-JNK, phospho-P65, and phospho-IκBα expression levels were upregulated by 60% in the kidneys with AKI, while ablation of mindin suppressed the activation of TLR-4/JNK/NF-κB signaling after IR injury as revealed by about 40% decreased expression levels of TLR4, phospho-JNK, phospho-P65, and phospho-IκBα. The gene discussed is SPON2; the disease is acute kidney injury.