Supporting the role of hyper-activated CeA PKC-δ positive neurons in the pathogenesis of CM (Additional file 7), chemogenetic inhibition of the CeA PKC-δ positive neurons via clozapine N-oxide (CNO, 5 mg/kg) application on day 10 and day 12 reversed the mechanical threshold in NTG-injected mice to the level comparable to Ctrl mice (Fig. 5D left), but the anxiety-like behaviors were unaffected (Fig. 5D right). This evidence concerns the gene PRKCD and Anxiety.