To further validate whether sensitization of the CeA PKC-δ positive neurons is responsible for the chronification of migraine, we applied chemogenetic activation of the CeA PKC-δ positive neurons by repetitive infusion of CNO in naïve mice pre-treated with AAV5-DIO-mCherry or AAV5-DIO-hM3Dq-mCherry virus (Fig. 7A). The gene discussed is PRKCD; the disease is migraine disorder.