GPC3 and liver cancer: To elucidate the functions of the three cell types, we first evaluated the target-mediated CAR-dependent cytokine production of iCAR-TCTL and iCAR-TILC by co-culturing the cells with SK-Hep-1 (a liver cancer cell line) transduced with GPC3 (SK-Hep-GPC3) as a stimulator.