GSEA showed enrichment for multiple relevant ontologies (Figure 7A), including IL-6 signaling, an important component of the SASP (22, 23); TGF-β signaling, an inducer of senescence and renal fibrosis; WNT signaling, crucial in cell fate decisions by regulating p53 (24); and PDGF signaling, important in myofibroblast differentiation and underpinning senescence in healthy wound healing (5, 25–27). This evidence concerns the gene TP53 and renal fibrosis.