Immunofluorescence staining showed that the staining signals of HSV-1 gD, 4-HNE, and CD11b+ monocytes/macrophages were progressively increased with increasing infection times (Fig. 7B to G), confirming that HSV-1-induced ferroptosis is tightly associated with the process of viral encephalitis. This evidence concerns the gene ITGAM and viral encephalitis.