HSV-1 infection at a multiplicity of infection (MOI) of either 0.1 or 1 increased with increasing durations, as determined by measuring the mRNA level of HSV-1 glycoprotein D (gD), gD fluorescence staining, and observing the cytopathic effect (see Fig. S1A to D in the supplemental material). Here, ACKR1 is linked to infection.