We also expanded our investigation to physiological NETs-inducers: IL-8 a key mediator associated with inflammation (Yang et al., 2020); TNF-α is a prominent inflammatory cytokines involved in various auto-inflammatory conditions (Turner et al., 2014); and TGF-β activates neutrophils in cancer (Fridlender et al., 2009). This evidence concerns the gene CXCL8 and cancer.