The cluster 2 showed elevated stromal and immune activation and was mainly enriched in some important tumor-related signaling pathways such as Notch, Gnrh, and MAPK signaling pathways, which had been suggested to be closely associated with tumor occurrences (Kranenburg, 2015; Lajko et al., 2019; Tang et al., 2021). The gene discussed is GNRH1; the disease is neoplasm.