We showed that FXS NPCs during early differentiation in vitro display specific gene expression changes, which are consistent with the impact of lack of FMRP seen on molecular and functional properties of neuronal cells and clinical phenotype (Davis and Broadie, 2017; Salcedo-Arellano et al., 2020; Utami et al., 2020). This evidence concerns the gene FMR1 and fragile X syndrome.