LYNX1 and fragile X syndrome: Reduced LYNX1 expression in FXS iPSC- and ESC-derived NPCs suggested dysregulated cholinergic signaling and motivated further comparison of gene expression in D7 NPCs derived from the three FXS (HEL69.5, HEL70.3, and HEL100.2) and the three control (HEL11.4, HEL23.3, and HEL46.11) iPSC lines with respect to putative changes in signaling pathways related to LYNX1. No specific pathway for LYNX1 was available and we explored gene expression changes of genes assigned Gene Ontology (GO) term GO:0007271, synaptic transmission, cholinergic, using the gconvert function in gprofiler2 (Figure 4A).