Disturbed cholinergic signaling in FXS NPCs was supported by increased expression of MYD88 and TRAPPC4. The inflammatory complex associated MYD88 is a target for nAChRs (Li et al., 2011), and TRAPPC4 encodes synbindin that is an organizer of cholinergic synapses (Zhou et al., 2021). This evidence concerns the gene TRAPPC4 and fragile X syndrome.