Based on this signaling pathway, we identified significant changes in TNF-α, IL-6, and matrix metallopeptidase 10 (MMP-10) gene expression when we performed RT-PCR in PC3 and 22RV1 prostate cancer cells, thus, we wanted to determine if there would be significant changes in expression in an in vivo mouse model. The gene discussed is TNF; the disease is prostate carcinoma.