Since the specific mechanism of COL6A2 in glioma is unclear, the purpose of this study was to comprehensively analyze the expression and significance of COL6A2 in glioma through the TCGA, CGGA and GEO databases, to verify COL6A2 expression by WB, PCR and immunohistochemistry and to determine the association of COL6A2 expression with prognosis in glioma. This evidence concerns the gene COL6A2 and central nervous system cancer.