In the current investigation, it’s the first time we find that the abnormal expression of Loxhd1b regulates BDNF, which can block the TrkB/ERK signaling pathway and cause auditory disorders; at the same time, BDNF positively regulates Loxhd1b. Therefore, we infer that the Loxhd1b gene is closely related to zebrafish hearing, which in turn, provided the potential prevention and treatment of patients with hearing loss in the future. The gene discussed is BDNF; the disease is hearing loss disorder.