Besides, the expression of immune checkpoint genes PD-L1 and CTLA4 in ARGcluster B were also notably higher than that in ARGcluster A (Figure 3D).These results further comfirmed the differences between two ARGcluster, such as biological characteristics and tumor microenvironment, higher infiltration of immune cells and higher ESTIMATE score were found in clusterB. This evidence concerns the gene CTLA4 and neoplasm.