To obtain information on whether VD may also have prevented inflammation in the kidney, we performed immunofluorescence analysis for renal IgG deposits and determined TLR-7 and TLR-9 mRNA levels as a putative measure of downstream activation induced by the deposition of nucleic acid–containing immune complexes (ICs) in animals with beginning nephritis (25–26 weeks) (70, 71). This evidence concerns the gene TLR7 and nephritis.