TLR9 and nephritis: To obtain information on whether VD may also have prevented inflammation in the kidney, we performed immunofluorescence analysis for renal IgG deposits and determined TLR-7 and TLR-9 mRNA levels as a putative measure of downstream activation induced by the deposition of nucleic acid–containing immune complexes (ICs) in animals with beginning nephritis (25–26 weeks) (70, 71).