Following the characterization of the PAO1 Tn-insertion pool, this library was used to determine which genes were conditionally important for survival in physiologically-relevant in vitro media (Belanger et al., 2020), in an in vivo murine abscess model (Pletzer et al., 2017b), and in an in vivo-like human skin organoid model (Wu et al., 2021). Here, SMOX is linked to abscess.