PKD1 and autosomal dominant polycystic kidney disease: We generated cysts from PT-derived Pkd1+/− (PH2) control cells and Pkd1−/− (PN24) ADPKD model kidney epithelial cell lines by growing the cells in Matrigel for a total of 12 days and treating these cells with forskolin plus a phosphodiesterase inhibitor (IBMX) from day 2–12.