We similarly tested whether these two ACLY inhibitors could reduce cyst area in IMCD-derived Pkd1−/− (ID1-3E5) cells grown in 3D Matrigel cultures treated with IBMX and forskolin and found that BA and SB-204990 both significantly reduced cyst size compared with vehicle (DMSO) (Supplementary Figure S2). This evidence concerns the gene ACLY and cyst.