To examine the role of ACLY more directly in governing ADPKD cyst growth in vitro, we generated stably transduced PH2 and PN24 cell lines using the pGIPZ lentiviral system to express either shRNA directed against mouse Acly (KD cells) or a non-silencing shRNA control (Figure 5). This evidence concerns the gene ACLY and autosomal dominant polycystic kidney disease.