Systemic endothelial dysfunction in 40-week-old BALB/c mice was associated with a tendency of lower eNOS expression, tendency of lower level of phosphorylated eNOS at S633 in the aorta (Figure 1B), and altered plasma levels of selected systemic endothelial dysfunction biomarkers sICAM-1, t-PA, Ang-1, ANXA5 (Figure 1C). Here, ANXA5 is linked to endothelial dysfunction.