The injection of 4T1 breast cancer cells did not affect plasma concentration of the following biomarkers of endothelial dysfunction: SDC-1, ESM-1, sVCAM-1, sICAM-1, sE-sel, t-PA, sP-sel, sTie-2, Ang-2, and sFLT (results not shown). The gene discussed is ESM1; the disease is breast cancer.