Although we confirmed the effect of knockout VASH1 on the promotion of glioma U-251 cell lines through in vitro experiments, its expression in the rest of the glioma cell lines was low, and its knockout VASH expression was found to be poor by qRT-PCR and Western-Blot detection, considering the need to further confirm this result due to our small VASH1 sequence. This evidence concerns the gene VASH1 and glioma.