VASH1 and neoplasm: Our findings show that VASH1 expression is highest in U-251 cell lines, and the ability to inhibit the migration and invasion of U-251-expressing cells is significantly increased, which is consistent with the results of Fu et al. In summary, we found that inhibiting VASH1 expression in in vitro experiments promotes tumor cell line migration and invasion, while in contrast to human LGG patients, high VASH1 expression is associated with poor prognosis, so we explored the mechanism of action of VASH1 in LGG through GO, KEGG, and GSEA.