AKT1 and cancer: Furthermore, the combined use of celastrol and triptolide has been shown to produce synergistic effects against various cancer models in vitro (H1299, H460, SKOV3, OVCAR3, Hela, SIHA, and SW480 cell lines) and in vivo studies (xenograft in mice) by inhibiting growth and proliferation through increasing ROS, G2/M phase arrest, decreasing Akt, surviving, and EGFR expressions [83].