ESR2 and melanoma: Now to understand whether ERβ may have a role in the EMT switch in melanoma cells of both sexes, in particular in acidic female melanoma cells expressing an increased level of ERβ in combination with a lack of EMT reprogramming, we used two approaches: (a) ERβ silencing assay and (b) cell sorter flow cytometry isolation of low and high ERβ expressing subpopulations.