Quantitative analysis of the mRNA levels of OPN splice isoforms by real-time quantitative PCR (qPCR) is shown in Figure 1C. The electrophoretogram highlighted that the expression levels of the OPNb and OPNc isoforms in adenomyosis were more obvious than those in the control group (p < 0.001). This evidence concerns the gene SPP1 and adenomyosis.