Consequently, to further elucidate the underlying mechanism of TRPV4-mediated cell morphological changes, we evaluated the effects of TRPV4 on the activation of cortactin and we found that the cancer metastatic sites formed by A375 cells show higher levels of cortactin phosphorylation levels compared with in situ cancer tissue (Figure 6A). This evidence concerns the gene TRPV4 and cancer.