TRPV4 and melanoma: To determine the specific role of TRPV4 in melanoma metastasis induced in A375, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) protein 9 (CRISPR/Cas9) lentiviral system was used to establish an A375 cell line with knocked out TRPV4, hence referred to as A375TRPV4−/− (Figure 2A).