When PINK1 siRNA, mdivi-1 (a mitophagy inhibitor) or BafA1 was supplemented to dampen the autophagy, both apoptosis and sensibility of multidrug-resistant cancer cells to B5G1 were counterbalanced which further highlighted the holding back effect of B5G1-induced mitophagy activation on its anti-HCC activities. This evidence concerns the gene PINK1 and hepatocellular carcinoma.