ATG5 and metabolic dysfunction-associated steatotic liver disease: Increased autophagic flux in HepG2 Cells and mouse hepatocytes in primary culture was also identified, and intracellular lipid content determined by bodipy staining was notably decreased by EGCG which was almost abolished after ATG5 or AMPK knockdown, indicating the crucial role of autophagy in NAFLD repression [100].