By recapitulating aberrant lipid metabolism involved in HCC development, we observed that liver-specific Prmt5 KO remarkably decelerated the tumorigenesis of Akt/N-Ras-transformed hepatocytes such as vacuolar denaturation, inflammation, and intracellular lipid accumulation (Figure 6C,D). This evidence concerns the gene PRMT5 and hepatocellular carcinoma.