Although HNRNPH1 silencing reversed the increase in PRMT5-ISO5 levels caused by IR while SRSF3 knockdown reinforced it, there was no significant difference in tumor growth between the experimental groups (Figure 5E and Figure S4A), indicating the prognostic value of PRMT5-ISO5 in HCC. Here, PRMT5 is linked to hepatocellular carcinoma.