In this study, our aim was to characterize DGCR8 microprocessor subunit in a series of thyroid lesions by genotyping DGCR8 recurrent mutation p.(E518K), to evaluate DGCR8 mRNA and protein expression by real-time PCR (qPCR) and immunohistochemistry (IHC), respectively. This evidence concerns the gene DGCR8 and thyroid gland disorder.