GLS-AS prevents GLS1 expression at the post-transcriptional level through the formation of double-stranded RNA with GLS1 pre-mRNA via adenosine deaminase acting on RNA (ADAR)/Dicer-dependent RNA interference so the expression levels of GLS-AS and GLS1 are negatively correlated in pancreatic cancer cells. This evidence concerns the gene GLS and pancreatic neoplasm.