Treatment with clomiphene (0.6 ± 0.06), combined KH + clomiphene (0.6 ± 0.05), combined KH + metformin (0.51 ± 0.04), metformin (0.45 ± 0.04), and KH (0.43 ± 0.03) significantly increased ERα mRNA expression (p < 0.05) compared with the untreated PCOS rats (0.05 ± 0.01). This evidence concerns the gene ESR1 and polycystic ovary syndrome.