Also, faster stress relaxed hydrogel (τ1/2) = 80 s) in both 2D and 3D cell culture environments enhances the proliferation of breast cancer cells (MCF-7 and MDA-MB-231) through phosphoinositide 3-kinases (PI3K)/Akt-p27Kip1 signaling axis in anchorage-independent cell growth [19,20]. Here, CDKN1B is linked to breast carcinoma.