Our previous study observed that BPAF up-regulated the mRNA levels of most targets associated with GPER1-mediated phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) and mitogen-activated protein kinase (MAPK) signaling pathways in ER-negative human breast cancer SK-BR-3 cells and induced the cell viability [24]. This evidence concerns the gene AKT1 and breast cancer.