This study found that BPAF markedly up-regulated the mRNA relative levels of some target genes related to ERα and GPER1-mediated estrogenic signaling pathways in the hypothalamus, ovary, uterus, liver, and kidney and BPAF at 20 mg/kg bw/day markedly increased uterine weight and uterine coefficient of nude mice with SK-BR-3 xenograft tumor. The gene discussed is ESR1; the disease is neoplasm.