Western blotting of 26 kinds of cell lines also indicated that the ACAP1 protein expression was high in Jurkat cells, an immortalized T lymphocyte cell line, while under-detectable in other cell lines, including kidney cancer (OS-RC-2, Caki-1, A-498), bladder cancer (RT112/84, J82, KU1919, BFTC905, UM-UC-3, 5637), liver cancer (SK-HEP-1, Huh-7, HepG2), colon cancer (HCT116, HT-29, SW480, SW620), lung cancer (A549, H1299, Calu-3, H1975) and esophageal cancer (KYSE-30, KYSE-140, KYSE-150, KYSE-410, KYSE-450) (Figure 1D). This evidence concerns the gene ACAP1 and urinary bladder cancer.