In accordance with the above observations, side-population cells with CS-like characteristics isolated from the non-small cell lung cancer (NSCLC) A549 cell line by flow cytometry also bore a hyperglycolytic profile, as indicated by the higher glucose uptake and lactate production, as well as by the higher expression of glycolytic enzymes (including PDK-1 and HK-1) compared to differentiated cancer cells [24]. The gene discussed is PDK1; the disease is cancer.