Spontaneous lung metastasis was analyzed using B16BL6 established from B16F10 melanoma cells and showed that the incidence of metastasis and the number of metastatic lesions were higher in drug-inducible endothelial cell-specific RAMP2 knockout mice (DI-E-RAMP2−/−), which could be due to the endothelial cells that were deformed and facilitating the infiltration of the inflammatory cells of the vessel walls. This evidence concerns the gene RAMP2 and melanoma.