Secreted levels of TNF-α and IFN-γ in the supernatant of NK-92MI cells with co-cultured overexpression of circRNF10 breast cancer cells were detected by ELISA (Figure 3K,L), which indicated that overexpression of circRNF10 caused enhanced cytokine secretion by NK-92MI cells, thus potentiating their cytotoxic effect. The gene discussed is TNF; the disease is breast cancer.