The OSNA cut-off levels were determined by Tsujimoto et al.: macrometastases was defined as >5000 copies/μL of CK19 mRNA, micrometastases as 250 to 5000 copies/μL and a value < 250 copies/μL correspond to absence of metastases or presence of isolated tumor cells (ITC) [14]. This evidence concerns the gene KRT19 and neoplasm.