We therefore examined in two highly motile GBM cell lines, DK-MG and SNB19, (1) the impact of elevated concentrations of glucose and inositol on the migratory activity by wound healing assays, (2) the subcellular expression patterns of SLC5A1 and SLC5A3 proteins by fluorescent immunostaining and (3) the GBM membrane permeability for glucose and inositol by osmotic swelling assay. This evidence concerns the gene SLC5A1 and glioblastoma.