Furthermore, flow cytometric analysis of the cell cycle revealed that the knockdown of MBNL1-AS1 resulted in significant S phase arrest (p-value < 0.05) (Figure 4C,D) In addition, EdU, Transwell, and scratch assays revealed that silencing the MBNL1-AS1 expression was associated with a significant increase in the proliferative and invasive abilities of the PC cells, both in vitro in PC cell lines and in vivo in a subcutaneous tumorigenesis mouse model (Figure 4F–K). This evidence concerns the gene MBNL1 and pachyonychia congenita.