To test whether HBV regulates WNT7B expression in HCC cells, we transfected Huh7 cells with the HBV replication plasmids, pCH9/3091 and PUC18-HBV1.3 plasmids, which contain 1.1 and 1.3 copies of the HBV genome, respectively. Here, WNT7B is linked to hepatocellular carcinoma.