In total, we retrieved 19 cuproptosis-related genes (CRGs) [5,13,14,15,16] based on published studies (including NFE2L2, NLRP3, LIAS, LIPT1, ATP7B, ATP7A, SLC31A1, FDX1, LIPT2, DLD, DLAT, CDKN2A, DBT, MTF1, GLS, GCSH, DLST, PDHA1 and PDHB) and found that 18 CRGs were differentially expressed (such as NFE2L2, NLRP3, LIAS, LIPT1, ATP7B, ATP7A, SLC31A1, FDX1, DLD, DLAT, CDKN2A, DBT, MTF1, GLS, GCSH, DLST, PDHA1 and PDHB) when comparing the gene expression between 1109 breast cancer tissues and 113 para-carcinoma tissues (Figure 1A,B). The gene discussed is GLS; the disease is breast carcinoma.