ANLN and breast cancer: Thus, using both ANLN-KO clones, derived from highly metastatic breast cell line (MDA-MB-231 and BT549) and anillin-overexpressing clones from poorly invasive MCF10AneoT cells, they have shown that anillin is necessary and sufficient to induce breast cancer anchorage-independent growth, motility and metastasis in vitro [111].