RBFOX3 and Alzheimer disease: To investigate the cell death and synaptic degeneration in the exhibiting circuit damage from early AD stage, such as retina-SCN, retina-dLGN, retina-IGL, retina-vLGN, and retina-SC pathway, we conducted immunostaining to detect NeuN, a marker of neuronal nuclei (Figure 8A), and SYN, a marker of pre-synaptic terminals (Figure 9A).