Lastly, we investigated whether DUSP4 overexpression affected AD-associated microgliosis and microglial activation, finding that DUSP4 overexpression reduced Iba1 and Cd68 mRNA levels and IBA1 immunohistochemical fluorescence intensity, in the dorsal hippocampus, in female but not male 5xFAD mice (Figure S10). Here, AIF1 is linked to Alzheimer disease.