To determine whether overexpression of DUSP4 would ameliorate AD-associated neuropathology as demonstrated following overexpression of the network driver VGF [14], we injected AAV5-DUSP4 or AAV5-GFP (control) into the dHc of 4-month-old male and female WT or 5xFAD mice (Figure 2C). This evidence concerns the gene DUSP4 and Alzheimer disease.