As for novelty in terms of GT, our analyses of bioenergetics and GTT allow us to suggest (with a certain degree of speculation) that especially in HFD conditions HGF/VEGF plasmid gene therapy of hindlimb ischemia can cause adaptive insulin resistance which induces mitochondrial biogenesis in skeletal muscle with consequent enhancing of regeneration energy supply. This evidence concerns the gene HGF and Insulin resistance.