The results of semi-quantitative RT-PCR assays confirmed the easily detectable overexpression and co-expression of a few selected examples of the CanCord34 genes of interest relative to the laboratory genes (i.e., TIGD5, EEF1D, PARP10, GPAA1, SCRIB, BOP1, PYCRL, and C8ORF73/MROH6) in the breast and other cancer cell lines (Supplementary Figure S7A,B). This evidence concerns the gene TIGD5 and cancer.