GSDMD and breast cancer: During an investigation involving signaling-dependent intramolecular conformational changes in GSDMD, an established mediator of pyroptosis with an emerging role in breast and other epithelial cancer types [57,58], we observed that the levels of GSDMD expression are widely upregulated in breast cancer and that its amplification and expression are closely paralleled with an oncogene, the HSF1 [59] (Supplementary Figures S1 and S2), which was already studied in the Kumar laboratory in the context of PAK1-signaling-dependent phosphorylation in breast cancer.