Additionally, ELISA results showed that IL-6, TNF-α, and TGF-β levels in serum from PBC mice increased along with decreased IL-10 levels relative to controls, whereas these findings were reversed in the PCS-treated group (IL-6, TNF-α, and TGF-β levels decreased, and IL-10 level increased), which showed levels similar to the control group (Figure 3C). Here, TNF is linked to primary biliary cholangitis.