Following LPS stimulation, we observed elevated mRNA levels of inflammatory cytokines (TNF-α, IL-8, MCP-1, IL-1β, and CX3CL1) and decreased mRNA levels of the anti-inflammatory cytokine IL-10 relative to levels observed in the BEC group and consistent with previous results and a clinical PBC phenotype. This evidence concerns the gene TNF and primary biliary cholangitis.