Notably, FBXO5 silencing induced the greatest increases in nuclear areas; however, silencing was associated with visual decreases in cell numbers (i.e., death) and agrees with FBXO5 being an essential gene as indicated within the Cancer Dependency Map (DepMap; https://depmap.org/; accessed on 29 September 2022) [58], whereas SKP2 silencing corresponded with the second largest increases but had minimal impact on cell numbers. The gene discussed is FBXO5; the disease is cancer.