In livers with established B16F10 luc2 melanoma metastases a significant difference in the numbers of Treg cells (P = 0.0368) and neutrophils (P = 0.0261) was found in livers of Lyve-1-KO in contrast to Ctrl (Fig. 5B, Additional file 1: Figure S6C). The gene discussed is LYVE1; the disease is melanoma.