To assess their roles in liver cancer tumorigenesis, a vector expressing constitutively active oncogenic Myc and AKT1, and single-guild RNA targeting Tp53 (sgTp53) was transfected into mouse liver cells based on a Sleeping Beauty (SB) transposon system via either HDVI or Epo (Fig. 1A, B). The gene discussed is EPO; the disease is liver cancer.