We therefore analysed all the tumors with image-based cytometry, using antibody sets to determine cancer cells (CD45−CD24+), tumor-associated macrophages (TAMs; F4/80+), cancer-associated fibroblasts (CAFs; CD45−CD90.2+), tumor-associated endothelial cells (TECs; CD144+) and tumor infiltrating lymphocytes (TILs; CD45+CD3+) (Additional file 1: Figure S14). The gene discussed is CDH5; the disease is cancer.