FMR1 and fragile X syndrome: The mean hair follicle FMR1 mRNA level using qRT-PCR in FXS participants was 0.055 ± 0.10 Delta Cq and 0.127 ± 0.11 Delta Cq compared to 0.165 ± 0.15 Delta Cq and 0.184 ± 0.14 Delta Cq for healthy participants at visit 1 and visit 2, respectively.