PALS1 and colorectal cancer: In a parallel approach, we tested another SMAP1-deficient colorectal cancer cell line (Fig. 5A), SW48 and observed indeed a similar behavior as with HCT116 cells: Deletion of Pals1 further reduced the cortical TJ marker ZO1 (Fig. 6A) and displaced PATJ and Crb3a from the junctions (Supplementary Fig. 5A).